|DATE||August 12 (Thu), 2021|
|INSTITUTE||University of Illinois at Urbana Champaign|
|TITLE||High throughput measurement of fatty acids on microbial colonies by optically guided MALDI-ToF-MS workflow for screening enzyme mutant libraries|
Engineering an enzyme often involves creation of many mutants followed by screening to identify a version that meets a goal. The bottleneck for the process is lack of high throughput assay that can directly measure the chemical product of enzyme activity. For example, directed evolution of an enzyme is limited by the number of samples and type of compounds that can be tested by the measurement instrument.